Although this mechanism of MT regulation by tau phosphorylation has been documented in vitro (Biernat et al., 1993) a more recent study in Sf9 cells showed that tau phosphorylation of several sites including Ser262 within the MT binding domain were necessary for the outgrowth of neurite like cell extensions induced by transfection of wild type tau (Biernat and Mandelkow, 1999).
The presence of the RB2 RNA-binding domain is necessary for the association of the MSL3 protein with the other complex subunits.
For the cross-linking of cell surface antigens, at least two binding domains are necessary.
The incomplete modifications fortuitously generated informative fragments that suggested that 1F3 and perhaps 7F1-9F1 of the gelatin-binding domain are necessary for the supportive activity of surface-adsorbed fibronectin fragments on fibronectin assembly.
This conservation indicates that the ATPase and peptide-binding domains are necessary for the survival of different biological species.
Second, mutational studies with GR indicated that neither the N-terminal regulatory or C-terminal ligand-binding domains were necessary to elicit the effects of As (Bodwell et al. 2004, 2006).
These results suggest that while Spire1C actin-nucleating activity may be unnecessary for mitochondrial fission when INF2 is constitutively active, INF2 binding to the Spire1C KIND domain is necessary for INF2 to maximally induce mitochondrial fission.
MMP-2 and MMP-9 (also named gelatinase A and B) are further characterized by the presence of three head-to-tail cysteine-rich repeats within the catalytic domain reminiscent of the collagen-binding type II repeats of fibronectin and this domain is necessary for the binding and cleaving activities of these MMPs [ 161, 165, 166].
The N-terminal Rel homology domain is responsible for dimerization and DNA binding while the COOH-terminal transactivation domain is necessary for positive regulation of gene expression.
Surprisingly, a fragment comprising only the RRM domain (aa 162 267) did not bind liposomes showing that the RRM domain is necessary but not sufficient for Nup53 membrane binding.
Moreover disulfide bond C233 C247 is located in the first Fibronectin type II domain and this domain is necessary for substrate binding.
