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The β-catenin binding domain is mapped to amino acid residues 535 to 636 of mXinα, which overlaps with the actin binding domains; i.e., the Xin repeats [9].
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For example, when a library made from the gene encoding the streptococcal receptin Mag, was panned against serum albumin, the binding domain was mapped to 42 amino acids (23).
Next, Mad and dTcf binding domains were mapped using truncation constructs (Fig. 5C).
The heparin-binding domain was mapped to the RRH 555-557) at the C-terminal region of GEP, implying that HS only binds to the full length GEP but not most of the granulin subunits generated from posttranslational proteolytic cleavage.
The ubiquitin-binding domain was mapped to a C-terminal four-helix bundle in ExoU [ 69], with PI4,5P2 synergistically enhancing the PLA2 activity of ExoU via a ubiquitin-related mechanism [ 70].
To restrict protein annotations to DNA-binding domains, each protein domain is mapped to its respective GO-annotation in Pfam and only considered when classified as 'DNA-binding'.
The ubiquitin-binding site of the Ubp-M BUZ domain is mapped to the highly conserved, concave surface formed by the alpha 3 helix and the central beta-sheet.
The p53 DNA-binding domain (IPR011615) is mapped to 'transcription regulatory region DNA binding' (GO 0044212).
The IGF2-binding domain has been mapped to domain 11, whereas M6P residues bind domains 3 and 9 (Braulke, 1999).
Mutational mapping-structure activity studies indicate that different residues of the ER ligand binding domain are involved in the recognition of structurally distinct estrogens and antiestrogens.
Here domain I (NADH binding domain) was used as ankerpoint.
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