The receptor-binding domain is localized in a position on the distal end of the molecule, closer to the 3-fold axis than anticipated, yet still in a position that would not impede these structural re-arrangements.
The Sp family proteins, containing the conserved DNA-binding domain, are localized primarily within subnuclear foci associated with the nuclear matrix.
Thus, the main cell binding, RGD-rich domain is localized within the 10th type III module, while heparin binding is mediated by modules 12 14 [49].
While the DNA-binding domain (DBD), composed of two zink finger domains, is localized within the highly conserved C region, the ligand-binding domain (LBD) is found within the E region.
The binding domain has been localized within the first exon of BCR and consists of at least two SH2-binding sites.
Since the Synapsin I ATP binding pocket is localized in the synapsin domain primarily involved in actin binding [69], [72], we investigated whether staurosporine binding is able to affect the synapsin I-actin interaction.
Here domain I (NADH binding domain) was used as ankerpoint.
Whereas p70 resembles a transcriptional activator, and its DNA-binding domain has been localized to its C-terminus, p80 does not seem to bind DNA and may be involved in interactions with other proteins [ 4].
In other figures, it was critical for us to be able to test just the motor domain to show that Rab6 regulation of KIF1C's MT binding is localized to the motor domain alone.
Arabidopsis TIFY proteins from group II lack a known DNA-binding domain but are localized to the nucleus, a pre-requisite for a protein to function as a transcriptional regulator [ 8].
Together, these data indicate that while multiple domains of Smad3 are capable of interacting with Sox17, the strongest binding is localized to the linker and MH2 regions from amino acids 146 425.
