NF-κB is such an example, as the NF-κB protein of E. lineata reflects the ancestral protein structure, in which the DNA-binding domain and inhibitory domain are contained within the same transcript, whereas these domains are split between two separate loci in N. vectensis.
Furthermore, the YL-1 alpha helical domain, which is thought to contain the DNA binding domain, is maintained in all intergenic splice products contained within YL-1 exons 4 and 5. To better understand the nature of YL-1's intergenic splicing, we searched the EST database for other alternative and intergenic YL-1 transcripts.
In this report, we have firstly expressed in stably transfected MDCK II cells a range of truncation mutants lacking up to 78% of the C-terminus of TSP1; these studies indicate that the N-terminal region containing the heparin binding domain is sufficient for basolateral targeting of TSP1.
The CysRS of B. subtilis (which also contains a DNA binding domain) is believed to play a role in initiating chromosomal replication [ 58].
Vpr55 91 sequence containing our newly identified PP2A1 binding domain is the most efficient transducing sequence.
In contrast to the ligand binding domain of the LDLR, which consists of 7 tandemly arranged so-called binding repeats, the chicken receptor's binding domain was found to contain 8 such repeats.
A protein, VC1059, annotated as a short chain dehydrogenase containing an NADPH binding domain, was identified in the active fraction.
In summary, a glycosphingolipid-binding domain is a flexible domain containing turn-inducing (Gly, Pro), basic (Arg, Lys, His) and aromatic residues (Phe, Tyr, Trp) (Refs 27, 54, 55, 56, 57).
Although no known DNA-binding domain is identified in its sequence, VosA contains a nuclear localization signal (NLS -pat7 (241PVKRQRT247) aNLS -pat765.2% likelihood of being locandzed in thasnucleus as predicted by PSORT II (http://psort.nibb.ac.jp).
The peptide-binding domain of HLA-DR2 transgenic mice is encoded by human sequences, while the membrane proximal portion containing the CD4-binding domain is encoded by mouse sequences (DRA1*0101: I-Eα and DRB1*1501: I-Eβ) [15].
The hormone-binding domain is located in the E region and contains, besides the binding domain, a dimerisation domain and a hormone-dependent transactivation function (AF-2).
