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To gain insight into H. akashiwo Trg1-RNA polymerase structure/function relationship as a possible transcriptional regulator of chloroplast genes, we constructed a model of the DNA binding domain for this protein.
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A 76-nt region at the 5′ end of the HEV genome has been identified as a binding domain for the ORF2 protein, and this region may be involved in the packaging of the HEV genome.
Both BPI and Pep 2.5 contain a high-affinity binding domain for the lipid A component of endotoxin.
Portions of receptors used correspond to extracellular domain for EGFR and IGF1R (receptor tyrosine kinases) and ligand binding domain for the other receptors (nuclear receptors).
LBP and bactericidal/permeability increasing (BPI) protein are comparable with respect to a high-affinity binding domain for the lipid A component of LPS.
The opposite can be observed after transfection of an antagonistic relaxin-2 analogue lacking the binding domain for the relaxin receptor RXFP1 [ 4].
Members of the kinesin family consist of a motor domain, a binding site for microtubules, and a specific cargo binding domain for the transport of different molecules and organelles.
Based on the reference genome protein structure prediction, the MYB-like DNA-binding domain for this gene spans amino acid positions 135 to 186.
In two instances, different groups have reported seemingly contradictory DISC1 binding domains for the same protein.
The independence of the DNA binding domains for the exonuclease and polymerase active sites varies significantly among DNA polymerases.
It is also interesting to note that this region is absent in the bacterial prototype of NEIL1, Nei, which insinuates that through evolution NEIL1 has acquired this binding domain for mediating multi-protein interactions in order to facilitate BER and combat the increased levels of oxidative stress that higher organisms face [ 40].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com