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The most conserved region is the binding domain for a heme cofactor.
Pho7 is classified as a putative transcription factor because it possesses a Zn2Cys6 binuclear cluster (ZC), a DNA binding domain for a number of transcription factors [ 31, 32].
The SOCS box was reported to act as a common binding domain for a large family of cullin-RING-based E3 ubiquitin protein ligase complexes.
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These results have significant implications for zinc finger design as they highlight the importance of considering affinity, specificity, and environmental requirements in designing a DNA-binding domain for a particular application.
MsbA is a 'half-transporter', comprising a TM (transmembrane) domain with 6 membrane-spanning helices, which are believed to contain the substrate-binding site, and a NBD (nucleotide-binding domain), for a total molecular mass of 64.5 kDa [ 4– 7].
Thus CD80 CD152 binding enhancement by γ4λhuCD152 (and possibly mAbs specific to this particular CDR2-like site) may be attributed to an extended protrusion of the CDR3-like region that facilitates CD80 engagement, or to a further segmentation that fixes the relative orientation of the binding domains for an additional spacing selectivity.
The Cbl family E3 ligases are composed of an amino-terminal tyrosine kinase binding domain for substrate recognition, a RING domain, a proline-rich domain, and a carboxy-terminal ubiquitin-associated domain [ 31].
Both BPI and Pep 2.5 contain a high-affinity binding domain for the lipid A component of endotoxin.
LBP and bactericidal/permeability increasing (BPI) protein are comparable with respect to a high-affinity binding domain for the lipid A component of LPS.
Class Ia kinases are multi-domain proteins having, in order from the N-terminus, a binding domain for p85 regulatory proteins, a RAS binding domain (RBD) as well as C2, helical (PIK) and catalytic domains.
Thus, Hep-HA hydrogel, where heparin acts as a binding domain for ADSCs and HA acts as a degradation site by cell secreted enzymes, was efficient for 3D culture of human ADSCs without any additional modification using biological/chemical molecules.
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