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EWS/FLI contains a carboxy-terminal ETS-family DNA binding domain contributed by the FLI portion, and an amino-terminal domain contributed by EWS [4].
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The EWS fusion proteins are potent transcription activators that require the EWS N-terminal domain and the C-terminal DNA-binding domain contributed by fusion partners [ 49- 54].
These findings further indicate that sequences covering nearly the entire 30-bp binding domain contribute to ATAF2 binding.
This indicates that the DNA-binding domain contributes to Chd1 function in vivo.
Tao, Y., Williams-Skipp, C. & Scheinman, R. I. Mapping of glucocorticoid receptor DNA binding domain surfaces contributing to transrepression of NF-kappa B and induction of apoptosis.
Here, the metal binding residues are contributed by two subunits.
In the crystal structure of the P-element DNA binding domain, this loop contributes to DNA binding specificity by making minor groove contacts (Sabogal et al., 2010).
It was also postulated that the conserved motifs lying outside the DNA binding domain may also contribute to the differential affinity displayed by ERF proteins towards the GCC box-containing promoters.
Peptide sequences outside the C-terminal WRKY domain contribute significantly to the overall strength of DNA binding; the N-terminal WRKY domain might participate in the binding process by increasing the affinity or specificity to their targets.
However, the biological function of this binding is not fully understood, and to date, the precise molecular mechanisms by which G9a and, in particular its ANK domain, contribute to de novo DNA methylation remain unclear.
It has previously been suggested that the microtubule binding domain of p150 contributes to processivity by augmenting interactions with the microtubule (King & Schroer, 2000; Culver‐ Hanlonet al, 2006).
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