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This reinforces the notion of a more conserved N-terminal (DNA binding) domain compared to a variable C-terminus.
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However, the IGR between WD_0100-WD_0102 has only 4 total binding domains (compared to 8) and, due to it's larger size (545 bp) a binding site density 1/3 that of the putative ori (0.007 vs 0.020).
What is most surprising is that, 70% of the trans-acting factors corresponding to over-represented TFBSs in the boundaries of U-CGIs possess zinc finger (C2H2-type) DNA binding domains comparing to only 17% in control set (Table 4), and 74% of these putative binding sites are found in the evolutionarily conserved regions.
From ELISA results, most of all chemical compounds showed a similar or a little lower binding activity for lck SH2 domain compared to the lead compound, (R -rosmarinic acid.
The outer domain of gp120 is a relatively stable domain compared to the inner domain and bridging sheet at the CD4-binding site for the HIV-1 primary receptor.
All five P. infestans BBEs possess the three residues required for FAD cofactor binding in fungal glucooligosaccharide oxidases (GOOX, related to BBEs, Figure 5A) and show a good conservation of the FAD-binding and BBE domains compared to their plant and fungal counterparts.
We found that Early genes were significantly enriched in NF-κB binding sites occurring in evolutionarily conserved domains compared to genes in the Late group.
The deduced amino acid sequence showed 93% identity in the DNA-binding domain and 72% identity in the ligand binding domain when compared to Aplysia estrogen receptor.
The fact that PPARβ/δ has a relatively large ligand binding domain as compared to other PPARs [ 42] and nuclear receptors supports the idea that PPARβ/δ can accommodate more than one endogenous ligand.
The number and lengths of the exons and the locations of the exon/intron boundaries were very similar to cortactin, especially in the exons that encode the actin-binding domain (compare [see Additional file 1] and [see Additional file 2]).
Interestingly, this association for DSS became non-significant when tumors harbouring TP53 mutations affecting the p53 L2/L3 DNA-binding domains were compared to those with wild-type or TP53 mutations outside the L2/L3 domains (p = 0.095).
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