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The most relevant example is the second generation Src kinase reporter [18], which like ROZA incorporates an SH2 phospho-tyrosine binding domain, and also undergoes a phosphorylation dependent decrease in FRET.
This included almost all of the cellulases with a CBM1 cellulose binding domain, and also the auxiliary proteins swollenin, CIP1 and one GH61 polysaccharide monooxygenase.
Kirrel3 A, but not Kirrel3 B, is predicted to contain a SH3 amphysisin binding domain and also a PDZ binding domain.
All three ERRs show close sequence identity to the ERα DNA binding domain and also feature a conserved C-terminal domain with a putative ligand binding domain and interaction surfaces for coregulators, and a less conserved N-terminal domain (Giguère 2002).
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The central and most conserved C domain contains the DNA binding domain, and it also mediates receptor dimerization.
The technique can be used to identify bacterial receptins and identification of their minimal binding domain, and but also to identify epitopes recognised by antibodies.
RHAMM has two microtubule binding domains and is also capable of binding to actin filaments thus potentially having a role in tumour cell motility.
The N-terminal regulatory region of PKD enzymes contains a DAG binding domain and direct binding of DAG also contributes to PKD1 activation [7] as well as regulating the spatial location of PKD enzymes within cells [8 12].
Additional residues lying within the preprotein binding domain and the C-terminus are also protected from exchange upon dimerization, indicating linkage to a conformational transition of the preprotein binding domain from an open to a closed state.
BORIS can interfere with CTCF functions in cancer cells not just by virtue of having the identical ZF binding domain and overlapping DNA binding specificity, but also due to its distinct amino- and carboxy-termini that likely confer a discrete set of molecular functions [2].
The extracellular N-terminal region of EGFR contains the ligand-binding domain and is also glycosylated.
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