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Because a TF's binding does not necessarily suggest function, additional functional data is obviously useful to further improve the model's performance.
However, because TF binding does not necessarily imply transcriptional regulation, it is also important to further extend computational methods to incorporate other, functional data, such as gene expression or protein level (time series) measurements.
While some mixed binders such as the 1918 HA variant A/New York/1/1918 or A/Hong Kong/213/03 (H5N1) virus do not transmit efficiently [13], [30], other mixed binders like A/Texas/36/91 (H1N1) virus do spread efficiently [13] thereby deomonstrating that mixed binding does not necessarily disqualify a virus from transmission.
A limitation of this study is that ligand binding does not necessarily indicate agonism of the receptor, leading to transcriptional events.
H3K4me3 marks the promoter regions and corresponds to RNAP II binding sites, however, RNAP II binding does not necessarily mean gene transcription actually occurs.
Although early work placed the episodic buffer among executive functions organized in the frontal lobes [ 51], later work has shown that multimodal information binding does not necessarily load on executive functions.
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For heterodimer formation, EMSAs with all eight possible combinations between AbdA and Exd fusion proteins showed that the protein topologies providing the highest levels of monomer binding did not necessarily yield to highest levels of heterodimeric complexes.
In addition, the above technologies only reveal TF binding, which does not necessarily suggest regulatory function of such binding.
In the concrete binding situation many external impacts will influence the folding of the aptamer, so that the structure of the highest binding affinity does not necessarily correspond to the structure yielding minimal free energy.
Unlike free ligands, ligand drug or drug carrier conjugates require congruency with target molecules for multivalent binding, which does not necessarily correspond to the maximal ligand density.
Since IgG binding alone does not necessarily reflect the quality of the subsequent antibody response, the subtype distribution of the opsonizing IgG molecules was also determined using a whole-cell ELISA.
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