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Our laboratory has used the DNA curtain technique to measure nucleosome binding distributions as a function of DNA sequence.
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As described in greater detail below, DNA curtain experiments have proven to be an efficient technique for determining binding distributions for a number of DNA-binding proteins.
As described below through illustrative examples, the binding distribution of a protein on a specific stretch of DNA under defined conditions is a direct readout of a portion of the binding landscape.
We evaluated experimentally the binding distribution of an RNA aptamer, hereafter referred to as GFPapt, on microcolumns that were filled with various amounts of GFP-immobilized resin.
Therefore, the histogram of binding probabilities can, for example, be considered as a mixture of two exponentially decreasing (no binding) and increasing (binding) distributions.
"Generally we need a binding agreement on a binding distribution of refugees between all member states based on fair criteria," the German chancellor told the Bundestag.
Interestingly, this binding distribution of SAFB1 is similar to the RNA-binding distribution of splicing factors SRSF3 and SRSF4 rather than hnRNP C protein; SAFB1 was initially classified as a novel member of the hnRNP protein family [ 6].
It had wide tissue distribution as well, which meant that a small change in the binding protein proportion would cause large variances in pharmacology.
We model the bin abundance distribution as a gamma distribution.
The analysis of the proton binding degree as a function of pH allows determining the distribution of dissociation constants.
To do this, political leaders must shift the perception of success as being a "politically binding deal as a opposed to a "legally binding" one.
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