Therefore, a mutational analysis of BMPR-IA was performed in order to see whether the functional epitopes, i.e. the main binding determinants for the interaction with both binding partners, are similar.
It is likely, however, that RNAP also provides binding determinants for the Mg2+ ion.
Further, the crystal structure of the BARD1 BRCT repeat uncovers a degenerate BARD1 BRCT phosphopeptide binding pocket with intact pSer interacting motifs but which lacks binding determinants for the pSer + 3 hydrophobic specificity pocket at the inter-BRCT repeat interface.
Other 2' substituted mannose motifs, such as GlcNAcβ(1 2 Man-, a glycan well-represented within Version 5.0 of the CFG glycan array and a binding determinant for the Lens culinaris agglutinin (LCA) lectin, were not bound by MVN.
The binding determinant for the NDP diphosphate group in these enzymes, namely a conserved arginine residue near loop C (e.g. Arg in 1N7G and Arg in 1A9Y), is also absent in hMAT2B.
Here we provide direct evidence that the β-sheet bundle of the C-terminal domain of NusG (NusG-CTD) has the binding determinants for Rho, proving the hypothesis described earlier [Mooney, R. A., Schweimer, K., Rosch, P., Gottesman, M., & Landick, R., (2009).
These structures represent a rare example of an ANL enzyme visualized in both conformations, and also reveal the binding determinants for both CoA and the hydroxycinnamate substrate.
Thus, natural molecular evolution as well as combinatorial evolution selected the same α-amylase binding determinants for completely different spatial frameworks.
The Bevacizumab VEGF binding profiles are consistent with the previous report that VEGF residues G88 and G89, but not I46 are important binding determinants for Bevacizumab [49], [51].
The presence of a non-active site, direct binding determinant for plg in the C-terminal region (Gly149-Lys158) of the uPA A-chain has been shown to exist on U937, a monocytic cell line [ 29], and we have previously shown that uPA and plg co-localise at the surface of breast cancer cells [ 28].
Thus the phosphate moiety within EBNA-1 NLS functions as a direct binding determinant for importin α5; phosphorylation of Ser385 within the NLS stimulates nuclear import by enhancing the binding affinity of EBNA-1 for the import adaptor importin α5.
