Therefore, a mutational analysis of BMPR-IA was performed in order to see whether the functional epitopes, i.e. the main binding determinants for the interaction with both binding partners, are similar.
Thus, natural molecular evolution as well as combinatorial evolution selected the same α-amylase binding determinants for completely different spatial frameworks.
Here we provide direct evidence that the β-sheet bundle of the C-terminal domain of NusG (NusG-CTD) has the binding determinants for Rho, proving the hypothesis described earlier [Mooney, R. A., Schweimer, K., Rosch, P., Gottesman, M., & Landick, R., (2009).
The Bevacizumab VEGF binding profiles are consistent with the previous report that VEGF residues G88 and G89, but not I46 are important binding determinants for Bevacizumab [49], [51].
It is likely, however, that RNAP also provides binding determinants for the Mg2+ ion.
Further, the crystal structure of the BARD1 BRCT repeat uncovers a degenerate BARD1 BRCT phosphopeptide binding pocket with intact pSer interacting motifs but which lacks binding determinants for the pSer + 3 hydrophobic specificity pocket at the inter-BRCT repeat interface.
By combining binding determinants from both substrates into a single molecule, the effective affinity and selectivity for the target is dramatically increased.
Peptide arrays further identified a major binding determinant for PKAc was located in exons 5 and 6 of AKIP 1A (Fig. 5A).
In 1998, Diep and colleagues demonstrated that the GPI anchor of these target proteins was an important binding determinant for aerolysin (53).
Thus the phosphate moiety within EBNA-1 NLS functions as a direct binding determinant for importin α5; phosphorylation of Ser385 within the NLS stimulates nuclear import by enhancing the binding affinity of EBNA-1 for the import adaptor importin α5.
