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Collectively these biochemical and SPOT array experiments indicate that AKAP2 binds directly to the C-terminus of AQP0, in a manner that involves binding determinants between residues 238 and 246 of the channel.
VHL has two well-defined TRiC binding determinants.
This combined approach led to useful insights into the binding determinants of thaperoxides to the malarial enzyme.
Thus, natural molecular evolution as well as combinatorial evolution selected the same α-amylase binding determinants for completely different spatial frameworks.
These structures represent a rare example of an ANL enzyme visualized in both conformations, and also reveal the binding determinants for both CoA and the hydroxycinnamate substrate.
To understand the binding determinants of the different analogs, hydrogen bonding and van der Waals contributions, and water molecule bridging in the EGFR analog complexes were analyzed.
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We found that purified ComA forms a dimer in solution, and we propose a model for how two dimers of ComA bind to an odd number of DNA-binding determinants to activate transcription of target genes.
We found that ComA binds a degenerate tripartite sequence consisting of three DNA-binding determinants or "recognition elements".
However, their IgE-binding determinants remain unknown.
Higher eukaryotic RSC lacks the specific DNA-binding determinants found on yeast RSC, and evidently Gal4 works in those organisms despite whatever obstacle broadly positioned nucleosomes present.
Mutagenesis studies performed on FSLLSH with natural and non-natural amino acid substitutions led to the identification of critical EPO-binding determinants, and the discovery of new peptide ligands.
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