We analyze chimeras between the three Roc proteins to map binding determinants, and demonstrate that both the RING and NH2-terminus of the Roc proteins can influence Cullin binding.
Moreover, residue N of CBEL_CBM1-1 and S of CBEL_CBM1-2 are well exposed and spacially-aligned with the aromatic binding determinants, and thus could also participate in cellulose binding.
Each RNA structure appears to have distinct binding determinants, and they bear little resemblance to the rRNA binding-site for S15 [ 25, 26].
Observed interactions of the products with the dizinc metal center and active-site residues provide insight into the substrate binding determinants and catalytic mechanisms of these enzymes.
Studies on the molecular binding determinants and molecular dynamics (MD) simulations of Keap1 Nrf2 PPI suggested that the incorporation of two acetic acid side chains to 3 would provide favorable binding interactions with the Keap1 Kelch domain.
We present the crystal structure of the complex refined to 2.3 Å resolution, define the sugar nucleotide binding determinants, and discuss the implications for the design of specific inhibitors.
The amino acid sequence of VH3-CDR3, thypervariableble region which in most cases acts as a major binding determinant [6] [8], is a unique and distinctive feature of each member of the repository.
The in vitro selected consensus sequence is similar to the natural one, but is extended by an additional binding determinant, a Val, which increases the affinity twentyfold.
These experiments support the model that the F8 aromatic residue in the BAT domain is an important binding determinant for an as yet unknown protein that limits telomere elongation.
Wu RA, Tam J, Collins K. DNA-binding determinants and cellular thresholds for human telomerase repeat addition processivity.
