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Many researches have demonstrated the obvious correlation between the binding degree and pH levels by in vitro assays.
Talbi et al. found that wogonin had a very high protein binding degree (over 90%) with rat plasma [28].
The analysis of the proton binding degree as a function of pH allows determining the distribution of dissociation constants.
However, because of the large errors and low resolution of SDS-PAGE, the method cannot reflect the binding degree of drug and the application is rare.
As argued by Howson (2000) and Howson and Urbach (1993), the Bayesian's aim is not to determine an intersubjectively binding degree of confirmation, but to provide sound inference rules for learning from experience.
The following quantities can be evaluated based on the Tg composition profiles: binding degree, fraction of plasticizer hydroxyl groups forming hydrogen bonds to PVP repeat units, the fraction of plasticizer molecules crosslinking the polymer units by hydrogen bonding through two or more hydroxyl groups in their molecule.
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Therefore, conventional methods are difficult to determine the binding degrees of these components with plasma proteins.
The obtained binding degrees are in good agreement with experimental results although with quite small energy differences.
They suggested that the binding degrees were about 27% for human plasma and 70% for rat plasma.
The five components (danshensu, protocatechuic acid, protocatechuic aldehyde, caffeic acid and ferulic acid) in danshen injection had suitable binding degrees with BSA.
Guo et al. found that compound I and compound M identified in Rhizoma Chuanxiong had the similar binding degrees to HSA as two known active compounds, ferulic acid and 3-butylphthalide [59].
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