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The binding data were subjected to nonlinear regression analysis using a one-site equilibrium binding equation with Prism software.
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Data were subjected to pre-planned contrasts.
Data were subjected to t tests (between two groups).
The data was subjected to statistical analysis.
The binding data were zero adjusted and the maximum binding responses of the individual injections were normalized using the BIAevaluation software version 4.1.
All binding data are summarized in Table S1.
Data is subject to different classification levels.
To analyze relative binding, the signals were subjected to densitometry.
The 44 genes thus obtained (additional data file 1) were subjected to comparative genomics analysis for the presence of conserved potential Sox10 binding sites.
Similar nanomolar affinities were also measured in a conventional radioligand binding assay where the ligands were subjected to freeze-drying and reconstitution in assay buffer (data not shown).
Data from these subjects were subjected for further analysis.
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