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The last approach is similar to the fourth but instead of using in vivo Tup1 binding data we used the change in expression for downstream genes in a tup1Δ [7].
To analyze the binding data, we used equation (1), describing a 1 : 1 interaction between one TRAF2 monomer and one GSTP1-1 subunithethe binding sites being equal and independent (see Materials and Methods section).
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In order to extract out the more reliable TF binding data, we have used MacIsaac et al. [ 34] filtered data which identifies genes with conserved sequence elements among three Saccharomyces species.
A future task can be the inclusion of distal binding information provided by the Encode project in addition to the Encode data we used for proximal binding to the TSS.
To explore other interacting factors that did not have genome-wide binding data, we repeated the above analysis using motifs from the JASPAR database [36], in addition to the motifs in this dataset.
To compare behavioral observations and histaminergic receptor binding data, we compared the treatment groups using a one-way analysis of variance (ANOVA) when there was a significant p-value ≤ 0.05, according to the Neuman-Keuls multiple-range post hoc test.
Prompted by this comment, we have re-evaluated fitting the Hill model to the binding data and used an alternative binding model (in the text and Figure 2d).
Since we do not have NFkB binding data in lymphoma cells, we used NFkB binding data in lymphoblastoid cells from ENCODE [ 33] instead.
The averaged binding data were used to calculate the Kd.
A large set of quantitative peptide-HLA binding data was used as input to train the NetMHCpan method.
In fact, the use of structural methods in combination with binding data is promising, as shown by which peptide binding data is used to parameterize structure based scoring functions.
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