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Kinetic analysis of the obtained binding data was done via the "Scrubber" software package provided with the SPR instrument to determine the equilibrium dissociation constant (Kd).
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Otherwise, assuming the ITC binding data are correct, the LysM2 domain does not directly out-compete for chitin-induced responses in the assay, and something else must be going on.
A promising approach that does not require binding data is to use 3D structures of peptide:MHC complexes.
The binding data were zero adjusted and the maximum binding responses of the individual injections were normalized using the BIAevaluation software version 4.1.
All binding data are summarized in Table S1.
Another main feature of STAP is the utility of genome-wide binding data not only as binary indicators of TF binding regions, as been done by most existing studies, but also as quantitative measurements of the binding strengths.
All binding assays were done in duplicate.
All binding assays were done in triplicates.
Simple forms of terminology bindings, e.g. explicit binding of one data entry to a single term could also be done easily.
Plastic runners are the thin bindings you normally throw away after you're done with a model kit.
Their business is done by 'phone & otherwise informally; verbal agreements are binding.
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