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However, ChIP-chip binding data may not be functional in terms of transcriptional regulation.
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However, ChIP data may not be sufficient to identify direct targets because genomic binding can be fortuitous and unrelated to gene regulation.
Data may not be available, a paradigm suggested not appropriate.
Although repeats 7 and 8 are not necessary for binding, they are not inactive in binding (data not shown).
These models may reveal combined binding sites of a transcription factor and its co-factors [ 1] and can be used to identify binding sites in species for which experimental binding data is not available.
Reversing the titration did not appreciably alter the binding data (data not shown).
But mAb binding would provide considerable extra stability; heparin-agarose binding may not.
However, their amino-termini contained two conserved aspartic acid residues (Asp-11 and Asp-56) that may serve as phosphate acceptors and their carboxy-termini contained several conserved residues that may be involved in DNA binding (data not shown).
Overall, the data across laboratories indicate that changes in QNB binding may not be a robust response to developmental exposure to pyrethroids and that conditions may need to be more carefully controlled in order to observe changes.
Structure prediction using HHpred [ 55] suggested that the N-terminus may contain a TIM-barrel-like structure involved in B12 binding (data not shown).
The binding of E4.3 and J4.48 clone was comparably diminished (J4.48 binding data not shown).
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