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Thus, in this evaluation, no peptide-HLA binding data from the validation set was included in the training of the pan-specific predictor.
Therefore, we used a factor analysis approach using a principal component analysis (PCA, total variance used) as a reduction method [64] to combine binding data from the two radiotracers into a single factor.
A survey of the available experimental peptide-MHC binding data from the Immune Epitope Database (IEDB) [31] shows that only a minute fraction of the hundreds to thousands of MHC allotypes for each locus have sufficient experimental data to train a sequence-based model.
Saturation binding data from the temporal cortex were averaged from all seven experiments.
To further analyze this phenomenon, we obtained the genome-wide binding data from the ChIP-chip experiments of Harbison et al. (2004) and used these to predict target promoters, sorted by P value, for each TF analyzed.
Genome-wide ATF2 binding data from the ENCODE Project (ENCODE Project Consortium, 2011) was used to identify genes with ATF2 binding sites −500 bp to +275 bp from the transcriptional start site (TSS).
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To provide direct verification of the unique and specific role of the AHR protein in the results, we compared binding data from these cells with data from the Hepa-1c1c7 mutant derivative c35 cell line, which has a missense mutation in the Ahr gene.
The combined energetic and XPS analysis of various oxygenated species demonstrates that non-hydrated OH, which has been suggested, by analysis of O 1s core-level binding data from state-of-the-art XPS studies, to be an abundant surface species at water/Pt(111) interfaces during ORR, is in fact unlikely to exist at potentials relevant to ORR, although it might be found in other conditions.
It proved impossible to obtain quantitative binding data from any of the dihydrogen phosphate titration experiments, indicative of a complex equilibrium binding process (chemical shifts observed were too small, association constants <100 m−1, to be sensibly fitted within error).
Gao et al. found that binding data from 37 of the 113 TFs (33%) from Lee et al. (2002) had a significant relationship to gene expression patterns in a set of ∼750 microarray experiments available at the time.
The binding data from two independent experiments were analyzed by fitting to the 'one-site specific binding with Hill slope' equation in GraphPad Prism 5 (GraphPad Software).
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