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The competitive binding data for each ligand was tested for both one and two -site binding.
The binding data for each mAb are displayed in Figure S1 and expressed as half-maximal binding concentrations at equilibrium (K50), which approximate to the equilibrium dissociation constants [58].
The competition binding data for each compound are expressed as the percentage of the remaining bound tritiated ligand after the compound's incubation with the membrane.
To identify these genes we developed a metric that accounts for both the significance of the expression change and binding data for each gene (described in detail in Methods).
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a, SPR binding data for the interaction of WM-8014 wimmobilizedized KAT6A and KAT7 MYST domains.
Training data consisted of sequences and binding data for nonamer (nine amino acid) peptides.
Independent test data consisted of sequences and binding data for peptides of length ≤25.
a, SPR binding data for the interaction of WM-1119 wimmobilizedized KAT6A, KAT7 and KAT5, compared with the interaction of WM-8014.
Intriguingly, while both BLI and SwitchSENSE demonstrated efficient target recognition for Cas9 tracrRNA crRNA complexes and no recognition for crDNA equivalents, the binding data for crHyb varied between the two methods.
(2011) reported a comprehensive transcriptome analysis of human hematopoiesis, combined with sophisticated bioinformatics analysis and high-throughput DNA binding data for multiple transcription factors.
The ability to obtain structural and binding data for these PI3Kα mutants, together with their ATR-like inhibitor binding profiles, makes these chimeric PI3Kα proteins valuable model systems for structure-based inhibitor design.
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