The pool of unique peptides was randomly split into five groups with all HLA binding data for a given peptide placed in the same group (in this way, no peptide can belong to more one group).
The combined list was derived from All-ER binding data for MCF7 cell [ 21, 22], Fun et al 2010 in preparation].
a, SPR binding data for the interaction of WM-8014 wimmobilizedized KAT6A and KAT7 MYST domains.
The competitive binding data for each ligand was tested for both one and two -site binding.
For AP1, ChIP-Seq binding data were available for all three classes (not cancer, MET, and Non-MET cancers), for both FOS and JUN TFs.
The binding data show cooperativity for all duplex substrates (Hill coefficients are 2.2, 1.5, and 1.6 for the dsRNA, A-DNA, and B-DNA duplexes, respectively, Figure 5A ), which suggests that multiple molecules of D2 can bind to a single duplex substrate.
The data for all binding proteins were fitted with the surface heterogeneity model, describing immobilized ligand (B) existing in different forms with different rate constants for interacting with the analyte, represented as equation 1 [40].
Average enrichment metrics are summarized in Table 2 and the complete data for all protein binding sets are visualized in Figure 3.
The global fitting analyzes both association and dissociation data for all concentrations simultaneously using 1∶1 Langmuir binding model.
Unfortunately, equivalent binding data are not available for all of the TCR pMHC interactions for which both bound and free TCR structures are available.
