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In addition, our flow cytometry and DNA binding data demonstrated that DNA could specifically bind to sperm via mAb C from all tested species, from birds to mammals including human.
scatchard analysis of the binding data demonstrated that the cells bound between 4.5 and 27.5 fmol mg-1 protein with a KD ranging from 16 to 40 pM.
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Our binding data demonstrate that HIV-1 envelope protein gp140 enters cells by protein receptor mediated interactions that are regulated by the conformational state of the gp140 at physiological environment (pH and temperature).
Notably, this competition is alleviated by interspersed cytidines, allowing strong U2AF65 binding in the presence of hnRNP C. Our iCLIP and in vitro binding data demonstrate that hnRNP C blocks U2AF65 from a large number of binding sites.
ITC data demonstrated that binding affinities of AEBP2379 390 peptide to the RBBP4 with mutations Glu231Ala and Glu126Ala/Asn128Ala/Glu179Ala are reduced by 5- and 7-fold, respectively, compared with wild-type RBBP4 (Fig. S5A).
These data demonstrated that binding of these antibodies prevents association with the labeled probe.
Our data demonstrated that RPol II binding pattern around the gene transcription start site follows distinct patterns (Figure 2A), and our model is designed to jointly describe the number of RPol II binding fragments surrounding the TSS, including both promoter and transcript regions; this allows for a more accurate description of RPol II binding pattern features.
These data demonstrated that the Aβ/QD binding ratio is correlated with oligomer formation.
Data demonstrated that the H/H phenotype affects binding between MAD1 and MAD2, disturbing the activation of the MSC.
These data demonstrated that exosporium was involved in PLG binding to spores.
These data demonstrated that SOX4 may regulate ABCG2 transcription by binding to the site.
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