Sentence examples for binding cycle from inspiring English sources

The method consists in injecting sequentially increasing concentrations of the partner in solution, with only one regeneration step performed at the end of the complete binding cycle.

Using the serial processing instrument, we were able to analyze three samples in each 30-min binding cycle, thereby requiring approximately 24 h to screen an entire 96-well plate of conditions.

The sensor surface was then coated with a fresh liposome suspension for the next binding cycle.

Rmax was set as a local parameter to account for the changing ligand density due to the capture of fresh ligand in each binding cycle.

After each binding cycle, the sensor surface was regenerated to the original matrix by injecting 50 mM NaOH/isopropanol (6∶4, v∶v).

Their activity is regulated by a network of co-chaperones that modulate the ATP-dependent peptide binding cycle of the chaperones and/or facilitate a cooperation with other protein complexes, chaperones, or degradation systems [6].

Wt1 has been implicated in the regulation of target genes related to cell cycle progression [ 31, 32] and Cyclin E1 has been demonstrated to play a crucial role in the cell cycle by binding cycle-dependent kinase 2 (CDK2), which phosphorylates Rb, leading to transition from G1 into S phase [ 36].

The antibody surface was regenerated between binding cycles by injection of 10 m m glycine (pH 1.7).

For repetitive photomodulated binding cycles the samples were reincubated under visible white light until equilibrium was reached.

Sample binding consisted of 15 binding cycles of 10 μL, followed by 3 wash cycles of 10 μL 0.1% TFA and 15 elution cycles of 8 μL of 80% acetonitrile.

The family shares a common NBD architecture (the Bergerat fold) and has a necessity for dimerization coupled to large-scale conformational changes as part of their ATP-binding cycles.