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Binding curves were then fit to the equation where Kd is the dissociation constant, P0 is the initial concentration of the unlabeled protein, and L0 is the initial concentration of labeled protein.
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The curves were then integrated in order to calculate the area under the curve (AUC).
Survival curves were then estimated.
Growth curves were then plotted.
The kinetic curves were then plotted.
The boundary curves were then constructed by smoothing across the manifold using a further principal curve.
The individual mean curves were then averaged across conditions to produce ensemble curves.
Survival curves were then compared among these two prognosis groups).
The growth curves were then scaled as described above.
The survivorship curves were then compared using log rank tests.
Curves were then compared using the Cox's F-test.
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