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The binding curves were processed by double referencing and were globally fitted to a 1 1 binding model.
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Buffer subtracted curves were processed using the program Primus.
Time activity curves were processed by graphical methods 9, using the cerebellum as the reference region to obtain 'binding potential' for the radioligand at baseline and after drug administration 12.
The processed binding curves were fitted to the Langmuir model for a 1 1 binding stoichiometry.
The binding curves were generated assuming a single binding site curve fits using the Graph Pad Prism software.
However, binding curves were fit to a nonlinear curve model that considers binding plus linear non-specific binding.
In each case linear binding curves were observed.
The binding curves were fitted using nonlinear regression as above.
The binding curves were fitted as described above.
The binding curves were analysed in Prism 5 (GraphPad) using a single-site binding model.
All binding curves were normalized with a blank flow cell and a control curve of the empty analyte injection.
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