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All data points on each binding curve were performed in quadruplicate.
All data points on each binding curve were performed in triplicate and each 96-well plate also contained 3 6 determinations of total and non-specific binding.
For competition binding, all data points on each binding curve were performed in triplicate, and each 96-well plate also contained 6 determinations of total and nonspecific binding.
Receiver operating characteristic (ROC) curves were performed.
Single curves were performed in each segment.
Calibration curves were performed as described by manufacturer instructions.
Standard curves were performed for each individual core compound.
Growth curves were performed in duplicate.
All computations and curves were performed using STATA 12.
Growth curves were performed in a Bioscreen C instrument.
Kaplan-Meier survival curves were performed for each treatment subgroup.
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