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All binding curves were normalized with a blank flow cell and a control curve of the empty analyte injection.
Binding curves were normalized using a blank ligand channel and a parallel buffer injection prior to fitting to a 1 1 Langmuir isotherm using the ProteOn manager software version 3.1.0.6 (Bio-Rad).
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The curves were normalized by the 0-min value at cell center.
The curves were normalized with respect to the undetermined background and amplitude.
Curves were normalized on sample transmission; scattering from buffer sample (water) was subtracted.
The C-V curves were normalized to their respective accumulation capacitance.
Reaction curves were normalized fixing the maximum activity for each protein to 100%.
Growth curves were normalized to the CI at the last measured time point before compound addition for each well.
Melting curves were normalized and analyzed with MeltWin 3.5.
Melting curves were normalized by subtracting baseline slopes.
Force curves were normalized to the animal's body weight.
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