Sentence examples for binding curves were fitted from inspiring English sources

The processed binding curves were fitted to the Langmuir model for a 1 1 binding stoichiometry.

The binding curves were fitted locally and the equilibrium dissociation constants (KD) were calculated from mean values of the obtained rate constants.

The data were analyzed and the binding curves were fitted by a one-site receptor model using Graphpad Prism 5.0 program.

The binding curves were fitted as described above.

The binding curves were fitted using nonlinear regression as above.

The subtracted binding curves were fitted globally to a 1 1 binding model to obtain association (ka), dissociation (kd) rate constants and the apparent dissociation constant (KD).

However, binding curves were fit to a nonlinear curve model that considers binding plus linear non-specific binding.

Sigmoidal binding curves were fit to the data using weighted least-squares regressions, and the data are presented as fractions of maximal binding based on these fitted curves.

The data points of the binding curves were fit to the equation F = P/ Kd + P) to determine the dissociation constants by Origin 7.5 (Microcal Software Inc ., where F is the fraction of RNA bound, P is the concentration of protein, and Kd is the dissociation constant.

To determine the end-point dissociation constant (KD), the binding curves were fit with the following equation using KaleidaGraph software (Abelbeck software, Reading, PA): An Ellman's reagent assay was used to assess the presence of free cysteine versus intramolecular disulfide bonds in LigB-12 cysteine mutants.

Binding curves were fit to the single-site binding equation using Prism version 5.0c (Graphpad Software).