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Thermophoresis + T-jump data shown as binding curves were evaluated by a NanoTemper software and calculated Kd values for PAB036, PAB046, and PAB50 variants were 40 ± 6 nmol/L, 49 ± 10 nmol/L, and 10 ± 3 nmol/L, respectively.
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The column strengths and load axial shortening curves were evaluated.
Differences in survival curves were evaluated with log-rank tests.
Six standard curves were evaluated in six independent assays.
Differences between curves were evaluated by the log-rank test.
Differences between curves were evaluated by log-rank test.
Survival curves were evaluated using the Kaplan-Meier method.
Kaplan Meier curves were plotted and differences between the curves were evaluated with the log-rank test.
At each 0.5 Myr time step the distribution of LOESS curves was evaluated and the probability distribution was determined.
The progress curve was evaluated using GraFit 5 (Erithacus Software Ltd).
The volume curve was evaluated as described above.
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