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Competition binding curves were constructed and IC50 values calculated using a 4-parameter logistic fit (PRISM 5.0, GraphPad Software, San Diego, CA).
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In the neonatal cefotaxime study the PD target was set at > 50%T>MIC, and using MIC values from the literature for pathogens that may cause neonatal meningitis, with consideration of drug protein binding, simulated concentration-time curves were constructed for each individual.
Standard curves were constructed using dextran m.w.
Calibration curves were constructed for this purpose.
ROC curves were constructed for amenorrhoea above 42 days.
Both normalized and standard TDS curves were constructed.
Standard curves were constructed with glucuronic acid.
Standard curves were constructed for each RT-PCR assay.
Rarefaction curves were constructed using Mothur [49] with default parameters.
Species-abundance curves were constructed for each individual assemblage (sample).
All tested curves were constructed from vertically upright rectangles.
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