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Control sensorgrams were subtracted on line from HS sensorgrams, and the resulting binding curves were analyzed using the Biaeval 3.1 software.
Four scFv concentrations in the range of 10 10000 ng/ml were subjected to the four antigen-coating concentrations by ELISA, and sigmoid binding curves were analyzed by Graph Pad Prism software.
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The titration curves were analyzed with Origin X (MicroCal) with ones-site binding model, with the first injection point excluded.
Radioligand competition curves were analyzed by nonlinear regression using the commercial Grafit curve-fitting software (Erithacus Software, Surrey, UK), by fitting the binding data to the mechanistic two-state dimer receptor model [18], [19].
MLR curves were analyzed using randomization test.
Current-frequency curves were analyzed using exponential curve fitting.
Survival curves were analyzed using the log-rank test.
Kaplan-Meier curves were analyzed by a Log Rank Test.
Fluorescence curves were analyzed with the LightCycler software v. 4.0.
Survival curves were analyzed by logrank test (GraphPad Prism).
Lifespan curves were analyzed using Prism.
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