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Binding curves obtained from chemical shift titrations of the two well-resolved amino acids Asp and Ser yielded a dissociation constant for the interaction KD ∼7 10 μM.
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In the Fig. 1C, binding curves obtained for all selected PAB variants are shown, representing one of the performed experiments.
Remarkably, the concentration-dependent binding curves obtained for the binding of each peptide to dsDNA displayed significantly different shapes.
The O2 binding curves obtained are shown in Figure 2.
This slightly overestimated the non-specific binding curve obtained with the automated well chamber.
All association and dissociation curves were corrected for non-specific binding by subtraction of control curves obtained from injection of the different analyte concentrations through the blank flow channel.
The time-activity curves obtained from dynamic PET measurements of this nearly non-binding isomer [68Ga]d-7 demonstheted the large difference in tumor uptake (Figure 5).
Binding curves obtained when Cy5 was positioned at either the 5′ or 3′ terminus closely resemble single site binding curves, whereas binding of XPD to centrally positioned Cy5-labelled oligonucleotides appeared more complex.
f-d curves obtained from sTNP tip under N2 condition.
The stress strain curves obtained from the improved ZWT model show good agreement with the experimental curves.
Similar results were obtained by evaluating the empirical ROC curves obtained from the testing set.
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