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We compared the binding curves for two preparations of β2GPI and for β2GPI-DV in the absence and in the presence of the dimerizing antibodies.
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Figure 1c shows titration binding curves for three tight SYNZIP interactions and one weak interaction.
Competitive receptor binding curves To validate the ERα FP RAD system, competitive binding curves for five known estrogenic test compounds DES, E2, EE2, tamoxifen and coumestrol were constructed using the FP-based microplate reader assay, and these values were compared with the IC50 values obtained using the ERα FP setup in FIA mode.
Saturation binding curves for the three mERs are shown in Supplementary Figure SI-1, and competitive binding curves are shown in Supplementary Figure SI-2.
Figure 5b shows 16 graphs, showing the results for one typical protein kinase per graph, with each graph having three actual binding curves for the same three different drugs: bosutinib, dasatinib, and imatinib.
We compared the binding curves for each antibody format using three different densities of NIP10-BSA covalently bound to the chip surface.
Shown at the bottom right of Fig. 5a are schematic binding curves of four different protein kinases (for which the ligand has no affinity for one protein kinase).
(2011) also stated that "the dose-response curve for AMP/ADP-mediated protection against (de phosphorylation correlates with the binding curves for these nucleotides at the weaker, rather than the stronger, of the two binding sites".
Under these conditions, we found almost identical binding curves for oxFRPcc and dissociable FRPwt with a submicromolar apparent Kd (Fig. 5b).
Figure 5 shows representative binding curves for the CYbU, A6U and the ND7-550 interactions.
Bottom: binding curves for H2A- and H2A.Z-nucleosome core particles.
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