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The binding curve was generated by fitting the data simultaneously to one-site and two-site competitive binding models, and the F test provided a preferred model for the mean IC50 values (confidence interval 95%).
The Kd binding curve was generated using Prism 5.0 software with nonlinear one-site binding regression to give Kd = 10.5±2.5 nM.
A competitive binding curve was generated using Prism (v.4.0; GraphPad Software, San Diego, CA).
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The binding curves were generated assuming a single binding site curve fits using the Graph Pad Prism software.
The sensorgrams were corrected with the blank reference and the binding curves were generated by global and local fitting with ForteBio Data Analysis software package 7.0 using a 1 1 binding model when R values were greater than 0.95.
Binding curves were generated by nonlinear regression analysis using the ligand binding function in Sigma Plot (SPSS Inc., Chicago, IL).
The standard curve was generated in Prism (GraphPad) using the linear regression curve fitting model.
Standard curve was generated following the manufacturer's instructions.
A standard curve was generated using a linear curve-fit.
A standard curve was generated using 1,1,3,3-tetramethoxypropane 1,1,3,3-tetramethoxypropane
A standard amplification curve was generated for all the samples.
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