Sentence examples for binding curve using the from inspiring English sources

Titration data were analyzed with a quadratic binding curve using the program Grafit 5.0 (7).

Analysis of the binding curve using the one site binding non-linear regression model in GraphPad PRISM software (Ver. 4.0) yielded high binding affinity for both MsSCP-2 and AeSCP-2 (positive control).

Logarithmic median effective concentrations (LogEC50) were calculated from respective binding curves using the web-based BioDataFit software.

The equilibrium constant (k d) for each sdAb and conventional antibody was calculated from direct binding curves using the formula, y = (Bmax)x/ Kd+x) (Table 2).

The immunoreactivity (i.e. the antigen-binding properties) of DOTA-BR96 relative to BR96 was determined from a saturation binding curve, using BN7005 cells as the target antigen.

The dissociation constants (Kd) were calculated by fitting the concentration-dependent binding curve using a quadratic ligand binding equation.

Data from titrations to measure nucleotide binding were corrected for dilution and analyzed with a quadratic binding curve using Grafit software: where P and L are the total concentrations of protein and ligand, respectively, Kd is the dissociation constant, and Fmin and Fmax are the fluorescence intensities of the free and ligand-bound proteins, respectively.

The data were fit to a saturation binding curve using nonlinear regression analysis in GraphPad Prism.

The apparent dissociation constant Kd was calculated by fitting the data to a one-site-specific binding curve using GraphPad Prism 6 software and was determined from three independent experiments.

The IC50 value (half maximal inhibitory concentration) was calculated based on competition nonlinear binding curve using GraphPad Prism software Version 7.03.

The observed changes in fluorescence versus NADH concentration were fit to a hyperbolic ligand-binding curve using SigmaPlot 10.