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Close inspection of the data reveals that while IHF binds to the nonspecific duplex (R3, black) in a monotonic manner, the binding curve for the specific duplex (I1, red) appears to be biphasic.
This was identified by visually inspecting the disappearance of bands, as was as the point the binding curve for the diglycosylated PrPsc band intersected the background density of the particular blot.
As the PTB binding curve for the mBS2+3 mutant was the same as the triple mutant, it was concluded that the minimum combination of mutations required to reduce PTB binding were in BS2 and 3. Clearly, although mutation of BS2 and 3 reduced PTB binding, some residual binding activity remained (Fig 4).
The time-course binding curve for the covalent PPARγ ligand GW9662 at low concentrations is very similar to the slow binding curve of compound 11.
In contrast, the binding curve for the Ac84 polymer was shifted markedly to the right, and little siRNA complexation was observed at N/P = 10.
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Averaged binding curves for the interaction between Ad and varying CYP11A1 concentrations were fitted simultaneously using the 1 1 Langmuir-binding model.
Figure 5 shows representative binding curves for the CYbU, A6U and the ND7-550 interactions.
The theoretical y values calculated using the Hill equation:
Saturation binding curves for the three mERs are shown in Supplementary Figure SI-1, and competitive binding curves are shown in Supplementary Figure SI-2.
Raw ITC data and the integrated binding curves for the titration of complex 3 with arsenate and phosphate can be found in the Supporting Information.
Lendel et al. used a variety of additional biophysical techniques, including isothermal titration calorimetry, to generate binding curves for the aS CR interaction.
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