In summary the comparison of sequence-based prediction of STAT1 binding and of observed ChIP-seq tag counts indicates that the predictions are hampered by a high number of false positives.
Furthermore microscopic counts indicate that only 20 to 40% of the total flora can be cultivated.
Comparison of these proteins' in vitro binding sites with their in vivo binding sites indicates that PBM-derived specificitiesificanies caccuratelyely reflect in vivo DNA sequence specificities.
The increase in binding affinity at lower pH was greater for the Ubx optimal binding site than for other DNA binding sites, indicating that subtle sequence alterations in DNA binding sites may influence pH-dependent behavior.
Stereological cell counting indicated that GluR2+ neurons account for 57% of the total hilar neuron population.
Analysis of receptor binding by autoradiography indicates that after 2 days, the number of cells specifically binding [125I]iodo-hCG increasignificantlyntly during culture, as does the intensity of binding on receptor-bearing cells.
The binding to ssDNA indicates that the protein is correctly folded, as it can bind to its substrate.
In kayviruses, the homologs of the α-GlcNAc-binding RBPs exhibit higher variability than WTA-backbone-binding RBPs, which indicates that they determine the host specificity.
The fraction of closed rings increases with increasing [ATP], of which dependence is similar to that of ATP binding to TClpB, indicating that ATP binding induces an open-to-close transition during the ATPase cycle.
Epidermal growth factor (EGF) and GTPgammaS did not inhibit 125I-monitor peptide binding indicating that the binding sites are not EGF receptors or G protein-coupled receptors.
