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Intriguingly, we observe that in 54 out of the 69 cases (78.3%) the top-ranked motif is associated with one PWM of an interacting TF, indicating that TF binding could be mediated by other proteins.
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This Aiolos and OBF-1 independent silencing pathway could be mediated through binding of Ikaros homodimers at the λ5 promoter.
This may be due to secondary transcriptional effects, which could be mediated by binding of HuR to the mRNAs encoding transcription factors that regulate EphA2 expression.
NLG-1 association with UNC-49 could be mediated by binding to an intermediary Gephyrin-like molecule.
Such movement of CD45 to lipid rafts could be mediated by binding to raft associated molecules including CD26 or influenced by actin polymerization [82].
Mechanistically, this could be mediated by direct binding of NF-κB to the promoter of cell-cycle mediators, as it has been shown to transcriptionally regulate cyclin d1[ 38].
We then asked whether GATA3 and ESR1 downregulation could be mediated by MYC binding to their cis-regulatory elements.
Considering that often MYC deregulation causes transcription repression of its targets by antagonizing the transcriptional activity of MIZ137,38, we determined whether the MYC-dependent downregulation of GATA3 and ESR1 could be mediated by MIZ1 binding.
Thus, the deleterious dominant effect of the CUG expanded RNA could be mediated by CUG binding proteins [10].
It is also possible that tsRNAs action could be mediated through RNA binding proteins or other potential molecules.
This interaction could be mediated by other SLex binding Cell Adhesion Molecules such as P-selectin [55], [56] or I-type Lectins.
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