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The ankyrin repeats of G9a and Glp1 have been shown to bind specifically to H3K9me1/2 and it has been suggested that this binding could be involved in the recruitment of the enzymes.
We also tested the possibility that CDK2 binding could be involved in the regulation of p27T198A/G stability.
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Whether unidentified SNPs in these binding sites could be involved in driving the association remains to be determined.
As hypothesized for other known lncRNAs, specific transcriptional factors, able to act as both RNA and DNA binding proteins, could be involved in forming a molecular platform in chromatin contexts.
Previous observation that CNSs are rich in transcription factor binding sites (Baxter et al. 2012) and the lack of a single specific motif in the CNSs of H3K27me3-marked genesuggeststhatat such highly conserved transcription factor binding islands could be involved in recruitment of PRC2.
Moreover, structural considerations suggest the existence of a secondary, putative low-affinity binding site (LBS) located in a positively charged region close to the AdoMet binding site which could be involved in binding the capped RNA substrate (Egloff et al., 2007; Mastrangelo et al., 2007a; Dong et al., 2008; Bollati et al., 2009a,b).
As a result of TEM-EDX analysis, both cell-surface binding and bioaccumulation could be involved in Pb removal.
The DNA region we refer to as island 4 is positioned next to an estrogen responsive element (ERE) binding site that could be involved in breast cancer.
Some of these proteins have homology to nucleic acid binding proteins and could be involved with pre-mRNA splicing; others are uncharacterized.
Residues 305 314 on the surface of the first subunit are not in a position to interact directly with the binding pocket, but could be involved in mediating interactions between the two subunits.
Endothelin 2 (EDN2) is another example for a gene with a putative promoter HIF1 binding site that could be involved in the differential expression of genes in the CoCl2 treatment.
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