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This binding could be inhibited by heparin.
Importantly, Mtb-lux bound to SR-B1 transfected R6F cells and this binding could be inhibited by the presence of serum, LDL and BAL (Fig. 2A, C and D, right panels), which was due to direct competition for the receptor (Fig. 2B, right panel).
Moreover, 28 (39%) of the patients had antibodies with overlapping specificities, so that the binding could be inhibited by peptides with different ureido group-containing amino acids.
Similar to the mouse protein, binding could be inhibited by oxysterols that activate Hh signaling but not by those that cannot; 7TM site ligands also failed to compete for binding.
Binding could be inhibited by free 20(S -OHC and free 20(S -OHC, the ∼10-fold more potent andyne analog ofree(S)-OHC (Nachtergaele et al., 2012).
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The binding of I-factor H could be inhibited maximally by 70% with unlabelled factor H. The binding data indicated that the ovarian cells SK-OV-3, Caov-3, PA-1 and SW626 have the ability to bind soluble factor H and FHL-1 from the fluid phase even in the absence of initial C3b deposition.
In another study, Naparstek and colleagues indicated that the binding of antibodies to dsDNA could be inhibited by heparin [ 104].
For another 16 (22%) sera the binding to homocitrulline-containing peptide could be inhibited by the corresponding citrulline-containing peptide.
Of these, 16 (22%) sera showed such binding to citrulline-containing peptide that could be inhibited by homocitrulline-containing peptide.
Prothrombin binding and procoagulant activity of PS liposomes could be inhibited >80% when 15 mol% DSPE-PEG 2000 was used.
Further, the binding of Mtb-lux to SR-B1 expressing cells could be inhibited in the presence of an antibody against SR-B1 (Fig. 2E, right panel).
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