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Three CBM-variants from the selection towards xylan (X-2, X-6 and X-13), the wt CBM4-2 and a human IgG4-specific module (G-4) that served as a non-carbohydrate binding control for the scaffold, were used in this work.
These affibody molecules have the same scaffold but different amino acids involved in binding and might be considered as negative not-IGF-1R-binding controls for ZIGF1R:4551-GGGC.
The level of Caprin-2-AVP mRNA binding was quantified by comparing it to the signal detected with the non-specific binding control IgG performed simultaneously for each individual sample.
As positive controls for binding, two pan HLA-DR/DP binding 13 and 15mer peptides were included.
The number of cases on which NF-κB detection (both DNA binding and fold of activation of DNA binding) was completed with controls for comparison was 67 children (Fig. 2).
The algorithm also accepts degenerate nucleotides in sequences; in which situation the worst-case variant is used (strongest binding for control set and weakest binding for target set).
Neither of these SR proteins cross-linked to the antisense-exons used as controls for binding specificity (exon 22A and exon 24A).
Another peptoid, 9, that does not bind to either antibody was subjected to the same synthetic protocol to prepare 9-PEG2000 and 9-PEG5000, wereh wemployedoyed as controls for binding experiment of NMOP6 to anti-AQP4 human IgG1.
In order to test for the stabilization of H2Kb cell surface expression by external peptides, the H2Kb-restricted peptide SIINFEKL or a non-binding control peptide (NGLTLKNDFSRLEG) were added for 24 h to cell cultures.
Mice were dosed with either 3 mg kg−1 of SGN-75 or a non-binding control ADC every 4 days for a total of four doses starting when tumour volumes reached 100 mm.
We also assessed disease/trait overlap using the central 200 bases of each binding site to control for differing size of binding sites and found very similar results (Additional file 1: Table S6).
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