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Samples for NMR analysis of ligand binding contained 75 µM 15N-rRKIP in 50 mM Tris-HCl pH 7.4 containing 100 mM NaCl and 7% D2O.
"Protein binding" and "ATP binding" contained the highest number of predicted peptides in the Molecular Function category (Fig. 2).
Fourteen of 19 (74%) malignant tumours and 17 of 49 (35%) benign tumours exhibiting high affinity binding contained GnRH-like immunoreactivity, suggesting that this system may be involved in prostatic epithelial cell growth in vivo.
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The active site for zinc binding contains two cysteine residues.
The most abundant group associated with lipid binding contains four transcripts comprised of nine EST and is homologous to mucins.
Strikingly, nearly all the 8-mers that are associated with RNAP binding contain the CpG dinucleotide while virtually none of the remaining 8-mers contain a CpG.
For example, external loops thought to be responsible for phage binding contain many more positively selected sites than the internal β-barrel region (composed of strands).
Analyzed region in front of porcine OPN promoter showed that CpG 1st binding site contained the specific binding site sequence.
Binding reactions contained binding buffer (Promega), labeled probe, and REV-ERBα protein.
Without the Ley ligand, the hu3S193 binding site contained 4 ordered water molecules and a glycerol.
Control binding reaction contained MBP resin saturated with MBP protein only.
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