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Here, the suggested experimental design allows for the determination of binding constants within a couple of hours using standard instrumentation.
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Furthermore, the L2 exhibited high sensitivity and selectivity overall emission change of more than 100-fold fluorescence intensity enhancement towards Hg2+ ion with a 1 1 binding stoichiometry (1.938 × 103 M−1 binding constant within detection limit as low as 5.4 × 10−8 M) in water samples.
The calculated binding constants show a comparatively weak ligand-protein interaction, corresponds to other strong ligand-protein complexes like monoclonal antibodies with binding constants from 107 M−1 to 1010 M−10109].
The almost negligible values of the binding constants suggest a possible lack of hormonal activity.
These binding constants were within physiological ranges and suggest the LRR domain can bind multiple peptide ligands.
To make a comparison of TM binding to native and truncated actins in different activation states possible, salt concentrations were selected separately for each state, so that the binding constants were within the resolution of this assay.
The number of binding sites and binding constants were calculated using a double logarithm regression equation.
The binding constants were obtained using a 1 1 Langmuir model.
The binding constant is the product of a charge-independent quantity, the intrinsic binding constant, and a cooperativity function.
As a result, no binding constants could be determined from the NMR titrations.
The results showed a reduction in the binding constants with the presence of probes.
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