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The binding constants were estimated by the change of migration time of the analytes in the buffer solution containing different concentrations of SARS 3CL proteinase.
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CTV CZ could associate with C60 to form supramolecular complex with 1 1 molar ratio and the binding constant was estimated to be 5.0 6)×104 M−1 by fluorimetry.
Parameters of the binding reaction (n, number of binding sites; K d, dissociation constant) were estimated using the LIGAND v4.5 program (Dr PJ Munson, National Institutes of Health, Bethesda, MD, USA), and the data were plotted according to the method of Scatchard [ 30].
By equilibrium binding fluorescence, the cell surface dissociation constant was estimated to be 122 ± 16 nM for AF, 101 ± 17 nM for FA, and 37 ± 6 nM for AFA, which are slightly higher than the data from previous studies using fluorescent protein-conjugated ZEGFR:1907.
Can the diffusion constant be estimated from the signals?
After docking, binding energies and estimated binding constants were parsed from results files with the Unix programs egrep, awk, and sort.
The binding constants were measured from two sets of experiments.
The binding constants were calculated by Stern Volmer method.
The binding constants were calculated using Origin provided by ITC200.
EGF binding constants were based on experimental measurements.
The binding constants were obtained using a 1 1 Langmuir model.
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