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The quenching constants and binding constants were determined for pSCX4 FL and pSCX4-FL Cu2+ systems.
Apparent binding constants were determined and ranged between 1.22 and 23.96 μM−1.
Composition of the complexes formed was determined from mass spectrometry and the binding constants were determined from fluorescence titration data.
The binding stoichiometry was determined at peptide concentrations between 1 µM and 10 µM, while the binding constants were determined at a peptide concentration of 1 µM.
The data were analyzed with the ProteON Manager TM 2.1 software; binding constants were determined using the software's Langmuir model.
Kinetic binding constants were determined by surface plasmon resonance using a BIACORE X instrument (BIACORE, Uppsala, Sweden).
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The stoichiometry of the H-bonded complexes is found to be 1 1 (receptor cyanide) and the binding constants are determined to be in the order of 105 M−105
A plot of receptor concentration versus absorbance at 445 nm yields a binding curve, which was fitted in Origin using a 1 1 binding model described by Hargrove et al.[ 34] Anion-binding constants were determined by preparing solutions containing a 1 1 ratio of 3 and PV (at 50 μ m) in HEPES buffer, and then adding increasing amounts of either arsenate or phosphate working solution.
The binding constant was determined according to Stern Volmer equation based on the fluorescence quenching of HSA in the presence of ADNR.
The binding constant was determined by Stern Volmer equation based on the quenching of the fluorescence HSA in the presence of bromsulphalein.
Changes of enthalpy, entropy and total free energy, reaction stoichiometry and affinity binding constant are determined or calculated at a pH value where the TiO2 nanoparticles surface charge is positive and the alginate exhibits a negative structural charge.
More suggestions(18)
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binding profiles were determined
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binding constants were detected
binding regions were determined
binding assays were determined
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