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The data is somewhat parallel to the flow dialysis data that assume no interaction between EF-hands and, thus, the corresponding intrinsic binding constants were derived by statistics.
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Subsequently, their corresponding dissociation constants were derived.
The first step association constants are derived.
The binding constants were measured from two sets of experiments.
The binding constants were calculated by Stern Volmer method.
The quenching constants and binding constants were determined for pSCX4 FL and pSCX4-FL Cu2+ systems.
The number of binding sites and binding constants were calculated using a double logarithm regression equation.
The binding constants were calculated using Origin provided by ITC200.
EGF binding constants were based on experimental measurements.
The binding constants were obtained using a 1 1 Langmuir model.
Anion binding constants are given in Table 2.
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