The calculated binding constants show a comparatively weak ligand-protein interaction, corresponds to other strong ligand-protein complexes like monoclonal antibodies with binding constants from 107 M−1 to 1010 M−10109].
The inhibition constants show that both L-1 and D-1 were shown to have strong binding affinity with L-1 being more potent than its enantiomer by 165-fold.
Analysis of dissociation constants shows that ΔN159ChlH is able to bind Proto and MgProto with normal affinities; the FRET data in Figure 5(A) indicate that the N-terminal truncation has not impaired the binding of Gun4 to this subunit.
Additional kinetic modeling using these rate constants shows that the differences in the rates of chemical catalysis between the two substrates are sufficient to explain their differing steady-state kinetics without the need to invoke differential binding affinities.
Previous theoretical and empirical analysis of the relationship between w, the bleach spot radius;, the pseudo first-order binding rate constant; Df, the diffusion constant in the absence of binding; and koff, the unbinding (or dissociation) rate constant showed that, when and, reaction-dominant models are the most appropriate (Sprague et al., 2004).
We show that dsRNA-binding proteins are prominent autoantigens.
The equilibrium binding constants (Kapp) obtained by fitting the experimental points to the Hill equation (Eq. 1) are summarized in Table 1 and show that actin truncation reduced the isoforms' affinity by a factor ranging between 1.2 and 1.5.
Since the order of response currents at the same concentration of each drug reflected their binding constants, it was shown that binding capacity of an unknown drug candidate could be estimated by comparison of response currents between the leader drug and the unknown drug candidate.
The observed rate constants show a linear dependence on the concentration of GDP, indicating that the speed of the fluorescence response in the concentration range measured is controlled by the bimolecular binding step rather than a conformational change.
