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This corresponds to dropping all biochemical parameter values, time constants, as well as binding constants, from the differential equation models.
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We emphasize the importance of differentiating the ideal definition of the binding constant from the theoretically evaluated including the activity coefficient, γ, to take into account shifts from the ideality of the polymer in both aqueous and lipidic domains.
Under these conditions, the height of difference peaks is not a direct measure of Na+ affinity, so that we cannot deduce an apparent binding constant from the difference peaks at pH 6. Accordingly, we have omitted the estimate of the binding constant at pH 6 from Figure 6.
The calculated binding constants show a comparatively weak ligand-protein interaction, corresponds to other strong ligand-protein complexes like monoclonal antibodies with binding constants from 107 M−1 to 1010 M−10109].
Since standard determination of binding constants from on- and off-rates may not reproduce binding constants in solution [22], we assume that the conclusions drawn from the latter experiments have greater biological relevance.
A strong NADH binding in contrast to weaker NAD+ binding of the protein was inferred from fluorometrically determined binding constants for the dinucleotide cofactor.
Cooperativity between U2A′ and SLII binding to SNF is only marginal (α = 2; Δ g° = −0.5 kcal/mol), so it was not possible to reliably determine the bimolecular binding constant for the SNF/U2A′ interaction from these titrations.
To compare the binding affinity of CA04/09 and CA04/09mut1 HAs to a representative human receptor, we calculated the binding constant Kd' from the 6'SLN-LN binding curves (Figure 2C).
Experimental data on the binding constants were taken from the PDB database and from the articles [ 35– 35].
The binding constants were determined from the changes in the Q-band maximum of the porphyrazine spectra at various poly (G-C) and DNA concentrations.
From the binding constants the following pattern of affinities emerges: PP i > UTP > UDP > UMP ≈ UDP-Glc ≈ Glc-1-P ≈ GlcNAc-1-P > UDP-GlcNAc.
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